Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

MSL975060 Mapping and Delivery Guide
Perform tissue or cell culture techniques

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency MSL975060 - Perform tissue or cell culture techniques
Description
Employability Skills
Learning Outcomes and Application
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.
Prerequisites/co-requisites
Competency Field
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities
Slides
PPT
Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Interpret and schedule production requirements
  • Review client request and confirm quantity and nature of cells or tissue
  • Select appropriate media, materials, equipment and methods
  • Plan parallel work sequences to optimise production
  • Maintain a chain of custody, traceable to the worker, for all cells or tissues
       
Element: Work safely according to the legal and regulatory framework
  • Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment
  • Select, fit and use personal protective equipment (PPE)
  • Address hazards and incidents as they arise in accordance with workplace procedures
  • Safely dispose of biohazardous materials and other laboratory waste
       
Element: Assemble and maintain tissue culture equipment
  • Assemble, sterilise or decontaminate equipment according to workplace procedures
  • Perform pre-use and safety checks in accordance with relevant workplace and operating procedures
  • Identify faulty or unsafe components and equipment and report to appropriate personnel
  • Decontaminate area and equipment after use
       
Element: Prepare and test cell or tissue culture media
  • Confirm media specifications and processes/methods
  • Prepare culture media to suit client request
  • Sterilise culture media and check for sterility
  • Perform quality control checks to ensure that culture media is fit for purpose
  • Store culture media in accordance with specifications
       
Element: Obtain, monitor and maintain tissue or cell lines
  • Access the cell lines or tissue sample from fresh or preserved sources and prepare a culture
  • Select specified culture media and add any necessary growth agents or nutrients
  • Incubate cells or tissue in specified conditions
  • Inoculate the media with the specified amount of sample
  • Monitor growth of tissue or cell lines and products
  • Detect contamination/abnormalities and troubleshoot materials, equipment and techniques
  • Passage samples by subculturing to preserve or grow the line
  • Harvest tissue, cells or cell products to optimise yields
       
Element: Preserve cells or tissues
  • Select the appropriate preservation method
  • Preserve the cell lines or tissue in accordance with the method
  • Check preserved tissue or cell lines regularly to ensure viability is maintained
  • Maintain accurate records of batches of media
  • Maintain accurate records of active and stored tissue or cell lines
  • Complete records so that they are retrievable, legible and accurate
  • Ensure records conform to the information management, records, quality system and legal requirements
       

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)


Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Review client request and confirm quantity and nature of cells or tissue 
Select appropriate media, materials, equipment and methods 
Plan parallel work sequences to optimise production 
Maintain a chain of custody, traceable to the worker, for all cells or tissues 
Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment 
Select, fit and use personal protective equipment (PPE) 
Address hazards and incidents as they arise in accordance with workplace procedures 
Safely dispose of biohazardous materials and other laboratory waste 
Assemble, sterilise or decontaminate equipment according to workplace procedures 
Perform pre-use and safety checks in accordance with relevant workplace and operating procedures 
Identify faulty or unsafe components and equipment and report to appropriate personnel 
Decontaminate area and equipment after use 
Confirm media specifications and processes/methods 
Prepare culture media to suit client request 
Sterilise culture media and check for sterility 
Perform quality control checks to ensure that culture media is fit for purpose 
Store culture media in accordance with specifications 
Access the cell lines or tissue sample from fresh or preserved sources and prepare a culture 
Select specified culture media and add any necessary growth agents or nutrients 
Incubate cells or tissue in specified conditions 
Inoculate the media with the specified amount of sample 
Monitor growth of tissue or cell lines and products 
Detect contamination/abnormalities and troubleshoot materials, equipment and techniques 
Passage samples by subculturing to preserve or grow the line 
Harvest tissue, cells or cell products to optimise yields 
Select the appropriate preservation method 
Preserve the cell lines or tissue in accordance with the method 
Check preserved tissue or cell lines regularly to ensure viability is maintained 
Maintain accurate records of batches of media 
Maintain accurate records of active and stored tissue or cell lines 
Complete records so that they are retrievable, legible and accurate 
Ensure records conform to the information management, records, quality system and legal requirements 

Forms

Assessment Cover Sheet

MSL975060 - Perform tissue or cell culture techniques
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:


Assessment Record Sheet

MSL975060 - Perform tissue or cell culture techniques

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: